Sunday, November 7, 2010

pH level affecting the rate of enzyme activity Lab

Procedure:
  1. Dilute solutions of HCl and NaOH in different test tubes according to the table below, add 5 mL of H2O2 to each test tube, swirl to mix
  2. Set up a water displacing station: fill a water trough and graduated cylinder with water (to the top), invert the graduated cylinder into the water trough carefully (do not let air in).  Place a beaker underneath the water trough to collect displaced water.
  3. Insert a rubber tube into the graduated cylinder that is connected to a rubber stopper (will be used to cover Erlenmeyer flask)
  4. In an Erlenmeyer flask, add 5 pieces of filter paper (soaked in liver juice) to the bottom of the flask
  5. Add the first dilution into the flask, shake and cover the flask immediately, use a timer to record time needed for the reaction.
  6. Stop timer and record results when oxygen stops displacing water and when water stops overflowing from the water trough
  7. Repeat steps 2-6 for other dilutions 
Title: pH level affecting the rate of enzyme activity Lab 

HCL
0ml
3ml
1ml
Water
0ml
2ml
4ml
time
39’
1:07’
1:45’
Amount water displaced
0 ml
0ml
5ml



NaOH
1ml
2ml
3ml
1ml *2nd trail
Water
4ml
3ml
2ml
4ml
time
2:10’
3:00’
2:40’
2:21’
Amount of displaced
gas
153ml
15ml
5ml
167ml
water
115ml
11ml
2ml
220ml

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